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Paraformaldehyde-fixed, paraffin embedded (mouse brain
tissue); Antigen retrieval by boiling in sodium citrate buffer
(pH6.0) for 15min; Block endogenous peroxidase by 3%
hydrogen peroxide for 20 minutes; Blocking buffer (normal
goat serum) at 37°C for 30min; Antibody incubation with
(KCTD17) Polyclonal Antibody, Unconjugated (bs-16929R) at
1:400 overnight at 4°C, followed by a conjugated secondary
(sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain);
Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for
15min; Block endogenous peroxidase by 3% hydrogen
peroxide for 20 minutes; Blocking buffer (normal goat serum)
at 37°C for 30min; Antibody incubation with (KCTD17)
Polyclonal Antibody, Unconjugated (bs-16929R) at 1:400
overnight at 4°C, followed by operating according to SP
Kit(Rabbit) (sp-0023) instructions and DAB staining.
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